H4C6 Knockout cell line (PC-9)

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H4C6 Knockout cell line (PC-9) Order

H4C6 Knockout cell line (PC-9)
Catalog#
YKO-H1164
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
H4C6 Knockout cell line (PC-9)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
H4C6 Knockout cell line（PC9）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
H4C6
Gene ID
8361
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 1 (based on transcript H4C6-201)
Validation: PCR+Sanger Sequencing
H4C6 Knockout cell line (PC-9) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the H4C6 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PC9 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, H4C6 gene knockout H4C6 Knockout cell line（PC9） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
H4C6 Knockout cell line (PC-9)
H4C6 Knockout cell line (PC-9)
Catalog#
YKO-H1164
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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